By Richard Mayou; Michael Sharpe; Alan Carson
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Therefore, the research group concluded that the Diels-Alder reaction was not the major reaction mechanism to produce polymers in the oxidation of edible oil. 46 5 Use of 13C NMR Spectroscopy for Determination of Lipid Oxidation aliphatic CH2 triacylglycerol CH2 olefinic CH PPM 120 terminal CH3 triacylglycerol CH 110 100 90 80 70 60 50 40 30 20 Fig. 1 The proposed Diels-Alder reaction and the DEPT spectrum of a fraction of oxidized soybean oil containing 92% polymers (Hwang and Bakota 2015; Hwang et al.
When it is used for two dimensional (2D) NMR techniques with 1H NMR, such as 1H–13C heteronuclear single-quantum correlation spectroscopy (HSQC), it can give information on which proton is attached to which carbon. The distortionless enhancement by polarization transfer (DEPT), one of the 13C NMR techniques, is used to distinguish CH, CH2, and CH3 groups. The attached proton test (APT) technique can also distinguish between carbon atoms with even or odd number of hydrogens. Therefore, the 13C NMR spectroscopy and related techniques have been used for analyses of foods and agricultural products, as well as for lipids and lipid oxidation.
To overcome this problem, a benchtop NMR spectrometer that can perform the 2D NMR analysis in a short time has been developed (Gouilleux et al. 2016). Therefore, it is believed that the NMR method will be widely used to determine lipid oxidation and will be thought of as a standard method in the near future. Furthermore, the current criterion for the safety level of oxidized oil is the amount of polar compounds in oil, while it was reported that the amount of polar compounds could not be representative of the safety level.
ABC of psychological medicine by Richard Mayou; Michael Sharpe; Alan Carson